Artificial insemination (AI) and semen cryopreservation are the most widely used assisted reproductive
technologies (ART) in livestock farming. Bovine reproduction, particularly in developing countries,
relies heavily on assisted reproduction using AI with cryopreserved semen. During cryopreservation, the
sperm cells face plenty of cold-induced insults. Especially oxidative stress due to excessive Reactive
Oxygen Species (ROS) production, the sperm plasma membranes suffer a lot of damage. It leads to the
reduction in the quality of spermatozoa in terms of its motility and fertility. Additives are various
supplements added in semen extender to improve the freeze thaw sperm recovery and fertility. Majority
of the supplements have antioxidant property hence scavenging free radicals and preventing lipid
peroxidation. Semen additives are classified based on their chemical composition and their mechanism of
action. This review article will discuss about various semen additives used over the past decade for
cryopreservation of bovine semen.
22 Oct 2020
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